A Method for Nucleic Acid Sequence Detection

A Method for Nucleic Acid Sequence Detection

Improved method to capture target DNA/RNA using sequence-specific cross-linking oligonucleotide probes. This technology enables a unique approach to Single Nucleotide Polymorphism (SNP) detection, and has broad potential in hybridization based nucleic acid detection methods.


Accurate and sensitive SNP detection is important in numerous fields within the life sciences. Standard methods rely on the cumulative electrostatic interactions between probe and target nucleic acids, resulting in a low power to discriminate between SNP and wild-type sequence at any single nucleotide position. MU’s technology uses modified oligonucleotide probes to create covalent bonds only at targeted SNPS to enable a novel and improved method to detect and/or capture SNP-specific nucleic acid sequences.


  • Detection of Single Nucleotide Polymorphism/Variation


  • Covalent crosslink enables greater detection sensitivity/specificity
  • Compatible with any hybridization-based methods including nanopore and PCR

Additional Details

Owner: University of Missouri - Columbia

IP Protection Status: Pending Patent